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治疗感染症的分子诊断 在分子诊断工具的领域，随著技术创新的发展使投资亦活络，对生物技术和医药品、医疗机器等关联企业来说，形成重要的事业领域。最近检测一般的病原菌和新的病原菌，被迫须加上特徵，以促进排序、微阵列、微流体工学等领域进步的形式，聚焦于开发各种诊断平台。若能使新排序平台和重点照护检验(POC)分析实用化，将可大幅提升作业，亦可提高便利性和灵敏度，促进分子诊断的新发展，在医疗和医药品开发的领域达成卓越的成果，亦能大幅削减成本。 第1天 | 第2天 10月12日星期三 13:00 Conference Registration
Molecular Diagnostics For Infectious Disease 14:00 Chairperson’s Remarks 14:05 Next-Generation Sequencing for Infectious Disease Surveillance - from “Base Pair to Bedside” Dag Harmsen, M.D., Head of Research, Periodontology Department, University Hospital Münster Next-generation sequencing (NGS) has fundamentally altered genomic research. New developments will bring NGS costs and performance down to an everybody’s technology with extreme potential for ultra fast and accurate molecular bacterial typing as it provides the ultimate whole genome information. However, the current bottleneck in analysis, i.e. bioinformatics, needs to be overcome to make successfully the transition from data to knowledge in routine infectious disease surveillance. 14:35 Adaptation of Next-Generation Sequencing for Exploration of the Malaria Epigenome Richard Bartfai, Ph.D., Postdoctoral Fellow, Molecular Biology, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Exploration of epigenetic regulatory mechanism unique to Plasmodium falciparum, the causative agent of malaria, could provide novel targets for drug development. We have developed the Linear Amplification for Deep Sequencing (LADS) method that enables preparation of highly representative sequencing libraries from the extremely AT-rich P. falciparum genome. Using this novel method we analyzed the epigenome (ChIP-seq) and transcriptome (RNA-seq) of the parasite at unprecedented depth, during multiple stages of development. 15:05 The Transcriptional Landscapes of Human Pathogenic Fungi Revealed by Next-Generation Sequencing Kai Sohn, Ph.D., Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology Human pathogenic fungi are causing superficial infections of the skin but also life-threatening systemic diseases. To define the pathogenicity at the molecular level, information about the genomes and the corresponding transcriptomes is crucial. We applied next-generation sequencing for the qualitative annotation as well as for the quantitative analysis of the transcriptional landscapes in different Candida species that represent the most important fungal pathogens. 15:35 Refreshment Break - Networking with Sponsors 16:15 Sponsored Presentations (Opportunities Available) 16:45 An iPod Touch-Based Hand-Held Gene Analyzer for Pathogen Screening in Limited Resource Settings Syed Hashsham, Ph.D., Professor, Civil and Environmental Engineering, Michigan State University Currently available genetic analysis platforms are powerful but they are expensive and too complicated for use by non-experts. We have developed a small and low cost device, called the GeneZ(TM) analyzer, which is a small smart phone-driven (both Apple’s iPod Touch and Google’s Android based phones) genetic analysis platform capable of analyzing 64 assays using an LED-photodiode system. The functionality and versatility of the platform will be discussed. 17:15 Application of Intact Cell MALDI-TOF MS to the Identification of Bacteria: From Colonies to Clinical Samples Bernard LaScola, M.D., Ph.D., Faculty of Medicine, University of the Mediterranean, Marseille Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) on intact cells is on the front end of a new revolution in the routine identification of microorganisms in clinical microbiology laboratories. It provides results in a few minutes and by its accuracy has the potential to replace or at least complement all other methods for microorganism identification.
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