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为了机能性筛选的RNAi
RNA干涉(RNAi)广泛作为特别指定潜在性药剂标的与确认疗法、探求未知细胞路径、以及实施全生染色体组机能筛选的筛选工具。此筛选用来开发低分子干涉RNA(siRNA)和小发夹型核醣核酸（short hairpin RNA; shRNA），现在相当稳固且感受性高，已成为可靠性高、高处理的工具。此会议是针对做各种应用之体外及体内RNAi筛选的活用、适当的模式系统和试剂的选择、RNAi delivery、Off-Target effect、解决是伪阳性或阴性问题、改善品质管理和数据分析等进行讨论的活动。

11月3日 星期四

Assay design和设定

1:30 pm Chairperson’s Remarks

Christophe Echeverri, Ph.D., CEO & CSO, Cenix BioScience GmbH

1:40 Talk Title to be Announced

Alex Gaither, Ph.D., Research Investigator II, Developmental and Molecular Pathways, Novartis Institutes for Biomedical Research

2:10 Screening Approaches Towards Identifying Genes Associated with DNA Re-Replication in Cancer Cells

Scott Martin, Ph.D., Team Leader, RNAi Screening, NIH Chemical Genomics Center, NIH Center for Translational Therapeutics, NIH

The NIH Chemical Genomics Center has established an RNAi screening facility that performs screens in collaboration with investigators throughout the NIH intramural community. An initial genome-wide campaign involved screening for genes associated with aberrant DNA replication. Screening was conducted using libraries comprised of both pooled and individual siRNAs. Combining these approaches led to a thorough examination of genes associated with DNA replication and served as a way to compare the value of both platforms.

2:40 RNAi Screening Comes of Age: For the Love of My Target

Hakim Djaballah, Ph.D., Director, HTS Core Facility, Memorial Sloan Kettering Cancer Center

RNAi screening has offered the premise of performing several thousand simultaneous knockdowns leading to the discovery and validation of existing and novel targets. Several years on, has the technology matured enough to keep up with its premise?

3:10 Networking Refreshment Break in the Exhibit Hall with Poster Viewing

3:45 Speaker to be Announced

Sponsored by
4:15 Sponsored Presentation
Speaker to be Announced

5:45 End of Day

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