高容许量蛋白质精制

支持技术

1月11日～12日

要实现高容许量蛋白质处理，使各阶段效率化、确保品质、短期能获得需要的成果，需要各种手法及支持技术。该部会检讨在高容许量(HTP)中使精制工程最佳化时可利用的策略，同时监视蛋白质的处理工程，分析结果，以及表示蛋白质状态和生物活性的各种手法。此外，包含各种软件和LIMS（实验室信息管理系统）数据的继续收集和处理技术，再加上选择复制品（clone）和缓冲的最适化、标签的删除等见识，亦采用液体处理系统和微流体技术等容许量自动化、引进单回使用技术和一次性技术等主题。

各种见识和技术创新

OPENING KEYNOTE PRESENTATION: High-Throughput Screening for Chromatographic and Non-Chromatographic Protein Purification Methods

Steven Cramer, Ph.D., William Weightman Walker Professor, Chemical and Biological Engineering, Rensselaer Polytechnic Institute

FEATURED PRESENTATION: High-Throughput Cloning, Expression and Purification: An Industry Perspective

Andrew Fosberry, Ph.D., Manager, Expression and Fermentation Sciences, GlaxoSmithKline

Beyond the Plan, What Really Happens in an HTP Project: Case Studies from a Core Service Lab

William Gillette, Ph.D., Senior Scientist, Protein Expression Lab, SAIC-Frederick, Inc.

难处理的蛋白质高容许量之精制

High-Throughput Expression and Purification of Membrane Proteins

Filippo Mancia, Ph.D., Assistant Professor, Physiology & Cellular Biophysics, Columbia University

支持技术

Test Method Development for High-Throughput Processes

Steven S. Kuwahara, Ph.D., Principal, QC, GXP Biotechnology LLC

Protein Arrays for Assessment of Human Anti-Viral Antibody Responses

Jonathan S. Duke-Cohan, Ph.D., Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Institutes of Medicine

高容许量制程的开发

Identification of Chromatographic Steps for Protein Purification using Parallel Screening Methods

Kaushal Rege, Ph.D., Assistant Professor, Chemical Engineering, Ira A. Fulton School of Engineering, Arizona State University

IT Partnership to Enable High-Throughput Process Development

Paul Hanson, Ph.D., Staff Engineer II, Cambridge Biologics CMC Group, Millennium Pharmaceuticals

高容许量制程的自动化

Automating Protein Purification for Genomic Enzymology Research

Sirano Dhe-Paganon, Ph.D., Assistant Professor, Physiology, University of Toronto