基因、传染媒介（vector）、构造体，复制品（clone）的开发

与下游成功有关之上游的判断

1月9日～10日

对生物学疗法可利用之高品质蛋白质的需求已扩大到未曾有过的规模。机能性蛋白质的生产如要达成提升到比迄今更高水准的目标，需要工学(科学)和洞察(方法论)。生物疗法用蛋白质有效的发现之课题，是与科学及方法论都有关的主题。此部会沿袭上次生物疗法用机能性蛋白质产品之蛋白质发现研究时应用蛋白质工学有效的策略之主题，能学习到由经验丰富的研究人员发表现场的经验及应用技术、研究成果等。

基因的标的化

Efficient Optimization of Protein Expression Using High-Throughput Gene Synthesis and Screens

Jingdong Tian, Ph.D., Assistant Professor, Biomedical Engineering, Duke University

Semi-Automatic Cloning and Expression of Immunoglobulin Genes from Single-Plasma Cells for High-Throughput Generation of Monoclonal Antibodies

Masaharu Isobe, Ph.D., Professor, Molecular and Cellular Biology, Life Sciences and Bioengineering, University of Toyama

传染媒介（vector）的构成

The Use of a Combinatorial Recombinational Cloning Platform to Simplify Construction of Expression Vectors for Wide-Ranging Areas of Proteomic and Genetic Research

Dominic Esposito, Ph.D., Interim Director, Protein Expression Laboratory; Group Leader, Clone Optimization Group, Advanced Technology Program, SAIC-Frederick, Inc.

Parallel Construct Expression Evaluation Using a High-Throughput Process for Baculovirus-Infected Insect Cells

W. Clay Brown, Ph.D., High-Throughput Protein Lab, Center for Structural Biology, Life Sciences Institute, University
of Michigan

Re-Engineering Multicloning Sites for Function and Convenience

Hal Alper, Ph.D., Assistant Professor, Chemical Engineering, University of Texas at Austin

So Many Options, So Little Money: How to Choose the Right Vector and Screen for Functional Proteins

Bill Gillette, Ph.D., Senior Scientist, Group Leader, Protein Expression Laboratory, Advanced Technology Program, SAIC-Frederick, Inc.

开发transient expression system

Maximizing Recombinant Protein Expression Through Systematic Gene Design

Mark Welch, Director, Gene Design, DNA2.0

Engineering and Evaluation of Mammalian Secreted Protein Expression Library for High-Throughput Target Discovery

Tadas Panavas, Ph.D., Senior Scientist, Molecular and Protein Biosciences, Centocor R&D, Inc.

Development of Expression-Ready Constructs for Generation of Proteomic Libraries

Susan Celniker, Ph.D., Co-Director, Life Sciences, Lawrence Berkeley National Laboratory

Driving a Variant of the Methanol-Inducible AOX1 Promoter from Pichia pastoris with Glycerol for High-Level Expression of (Therapeutic) Proteins

Roland Weis, Ph.D., Head, Operations, VTU Technology GmbH, Austria

Influence of Histidine Affinity Tags on Protein Structural Order

Evelina Angov, M.S., Ph.D., Microbiologist, Malaria Vaccine Development, Walter Reed Army Institute of Research