宿主和平台的选择、设计、最适化

1月11日～12日

蛋白质的研究人员正面临要在更短期能大量生产蛋白质的课题。依情况有时需要新的技术和策略，然藉由使现有的供给源再生或再设计，亦有可能增加生产量。最重要的是选择发现蛋白质的宿主时要作出聪明且有效的判断。此部会检讨可能是宿主/系统最佳选择的最新数据和创新的技术，同时考量如何「挽救」现有系统，更有效改善机能，以及提高生产蛋白质的质和量之方法。

原核生物成果的最大化

Engineering of Escherichia coli for Endotoxin-Free Production of Recombinant Proteins and Plasmid DNA

Uwe Mamat, Ph.D., Research Professor, Center for Medicine and Biosciences, Research Center Borstel

Transient Expression of an E. coli Periplasmic Expression of Fab乫 Fragments at 2-5g/l

Mark Ellis, Senior Scientist, Protein Expression and Purification, UCB Celltech

Discovery Protein Expression Enabled by Pfenex Expression Technology™

Diane Retallack, Ph.D., Director, Molecular Biology, Pfenex, Inc.

扩大对真核生物的投资收益

Early Markers of Production Instability of Recombinant Chinese Hamster Ovary Cell Line

Ulrich Göpfert, Ph.D., Pharma Research and Early Development, Roche Diagnostics GmbH

Optimizing the Baculovirus-Insect Cell Platform for Recombinant Glycoprotein Expression

Donald Jarvis, Ph.D., Professor, Molecular Biology, University of Wyoming

The Baculovirus Expression Platform: Recent Advances & More Lessons Learned

James M. Groarke, Ph.D., Biortus Biosciences

Generation and Screening of Pichia pastoris Strains with Enhanced Protein Production by Use of Microengraving

J. Christopher Love, Ph.D., Associate Professor, Department of Chemical Engineering, Massachusetts Institute of Technology

加强发现高容许量

Maximizing the Success of Protein Expression in High-Throughput Fashion

Yvonne Franke, Ph.D., Scientist Technology, Structural Biology, Genentech, Inc.

Improving Protein Production in Insect and Mammalian Cells using Combinatorial Libraries of Expression-Enhancing Elements

Dominic Esposito, Ph.D., Director, Protein Expression Laboratory, SAIC-Frederick, Inc.

Self-Cleaning Fermentors and Auto-Inducing Media for High-Throughput Parallel Expression in 1–5 l Scale

Alvar Gossert, Ph.D., Investigator, Structural Biology Platform, Novartis Institutes for BioMedical Research