克服课题与探求解决方案

1月12日～13日

有必要治疗和诊断的大半蛋白质，很难在原有的宿主身上发现，存在折叠（folding）、溶解性和无法进行醣化作用（Glycosylation）等问题，而且对宿主都算是有毒性的东西。此部会介绍蛋白质科学领域的最新成果，发表克服这些课题的见解与解决方案。

重点发表

Protein Expression in Drug Discovery – New Challenges, New Solutions

Lorenz Mayr, Ph.D., Executive Director, CPC/EPP, Novartis Pharma AG

Enhanced Glycoprotein Production in HEK-293 Cells Expressing Pyruvate Carboxylase

Yves Durocher, Ph.D., Chemical Engineering, École Polytechnique de Montréal

因应溶解性、折叠（folding）、无法进行醣化作用（Glycosylation）问题的解决方案

Expression of Soluble Proteins by Linkage with Acidic Tags

Richard Discipio, Ph.D., Research Scientist, Torrey Pines Institute for Molecular Studies

Measurement of Protein Unfolding/Refolding Kinetics and Structural Characterization of Hidden Intermediates by NMR Relaxation Dispersion

Derrick Meinhold, Ph.D., Research Associate, Department of Molecular Biology and Skaggs Institute of Chemical Biology, The Scripps Research Institute

Effects of Cell Culture Conditions on Antibody N-Linked Glycosylation—What Affects High Mannose 5 Glycoform?

Efren Pacis, Biologics Pharma Technical Development, Genentech, Inc.

解决膜蛋白质的问题

Restrained Expression: A Method to Overproduce Toxic Membrane Proteins by Exploiting Operator-Repressor Interactions

Dinesh A. Yernool, Ph.D., Assistant Professor, Department of Biological Sciences, Purdue University

Reprogramming Chaperone Pathways to Improve Membrane Protein Expression in Escherichia coli

Francois Baneyx, Ph.D., Professor, Chemical Engineering, University of Washington, Seattle

因应蛋白质凝集特性描述（characterization）之分析法的课题和进步

Aggregates and Their Analysis: Some Perspectives and Lessons from Working with 100+ Products

John Philo, Ph.D., Vice President, Director of Biophysical Chemistry, Alliance Protein Laboratories

Addressing New Analytical Challenges in Protein Formulation Development

Speaker to be Announced

Characterization of Submicron Particle Distributions in Biologics Formulations

Flaviu Gruia, Ph.D., Scientist, Analytical Biochemistry, MedImmune

Characterization of Sub-Visible Particles in Therapeutic Protein Formulations

Tobias Frommknecht, Ph.D., Formulation Scientist, pRED, Hoffmann-La Roche Ltd